File name descriptions for modERN database
Alignments:
These files contain the sequence reads aligned to the genome with an alignment score greater than 30.
Alignment file for rep1: CEH-34_OP524_WA_LE_IP_GoatV-aGFP_Rep1.bam
Alignment file for rep2: CEH-34_OP524_WA_LE_IP_GoatV-aGFP_Rep2.bam
Alignment file for input: CEH-34_OP524_WA_LE_Input_NA_Rep1.bam
Control normalized signal:
Replicates and pooled replicates with input subtracted to view signal tracks in UCSC genome browser or IGB. Allows you to view signal above input.
Rep1 input subtracted:
CEH-34_OP524_WA_LE_IP_GoatV-aGFP_Rep1.tagAlign_VS_CEH-34_OP524_WA_LE_Input_Rep0.tagAlign.bw
Rep2 input subtracted:
CEH-34_OP524_WA_LE_IP_GoatV-aGFP_Rep2.tagAlign_VS_CEH-34_OP524_WA_LE_Input_Rep0.tagAlign.bw
Pooled replicates input subtracted:
CEH-34_OP524_WA_LE_IP_Rep0.tagAlign_VS_CEH-34_OP524_WA_LE_Input_Rep0.tagAlign.bw
Optimal IDR thresholded peaks:
Files give you the called peaks that passed IDR for both replicates
bigBed file is the called peaks that are above the Irreproducibility Discovery Rate (IDR) for both replicates for viewing in the UCSC or IGB genome browsers:
spp.optimal.CEH-34_OP524_WA_LE_IP_Rep0.tagAlign_VS_CEH-34_OP524_WA_LE_Input_Rep0.tagAlign.regionPeak.bb
BED file that can be used to call targets. Files give you the called peaks that passed IDR for both replicates:
spp.optimal.CEH-34_OP524_WA_LE_IP_Rep0.tagAlign_VS_CEH-34_OP524_WA_LE_Input_Rep0.tagAlign.regionPeak.gz
Peaks:
Files give you the called peaks even below IDR for both replicates>
BigBED files:
Allows you to view all called peaks (even below IDR) in UCSC or IGB genome browsers for pooled replicates and each individual replicate
Pooled replicate peaks:
CEH-34_OP524_WA_LE_IP_Rep0.tagAlign_VS_CEH-34_OP524_WA_LE_Input_Rep0.tagAlign.regionPeak.bb
Rep 1 peaks:
CEH-34_OP524_WA_LE_IP_GoatV-aGFP_Rep1.tagAlign_VS_CEH-34_OP524_WA_LE_Input_Rep0.tagAlign.regionPeak.bb
Rep 2 peaks:
CEH-34_OP524_WA_LE_IP_GoatV-aGFP_Rep2.tagAlign_VS_CEH-34_OP524_WA_LE_Input_Rep0.tagAlign.regionPeak.bb
BED files:
Allows you to visualize peaks in EXCEL for pooled replicates and each individual replicate
Pooled replicate peaks:
CEH-34_OP524_WA_LE_IP_Rep0.tagAlign_VS_CEH-34_OP524_WA_LE_Input_Rep0.tagAlign.regionPeak.gz
Rep 1 peaks:
CEH-34_OP524_WA_LE_IP_GoatV-aGFP_Rep1.tagAlign_VS_CEH-34_OP524_WA_LE_Input_Rep0.tagAlign.regionPeak.gz
Rep 2 peaks:
CEH-34_OP524_WA_LE_IP_GoatV-aGFP_Rep2.tagAlign_VS_CEH-34_OP524_WA_LE_Input_Rep0.tagAlign.regionPeak.gz
Read-depth normalized signal:
Wig files allow you to view signal tracks in UCSC or IGB genome browsers for pooled replicates and each individual replicate as well as input control. These wig files are scaled based on the number of reads.
Pooled replicate wig:
CEH-34_OP524_WA_LE_IP_Rep0.tagAlign_density.scaled.bw
Rep 1 wig:
CEH-34_OP524_WA_LE_IP_GoatV-aGFP_Rep1.tagAlign_density.scaled.bw
Rep 2 wig:
CEH-34_OP524_WA_LE_IP_GoatV-aGFP_Rep2.tagAlign_density.scaled.bw
Input wig:
CEH-34_OP524_WA_LE_Input_Rep0_density.scaled.bw
Signal of unique reads:
Wig files allow you to view signal tracks in UCSC genome browser or IGB for pooled replicates and each individual replicate as well as input control. These wig files are NOT scaled based on the number of reads.
Pooled replicate wig:
CEH-34_OP524_WA_LE_IP_Rep0.tagAlign_density.bw
Rep 1 wig:
CEH-34_OP524_WA_LE_IP_GoatV-aGFP_Rep1.tagAlign_density.bw
Rep 2 wig:
CEH-34_OP524_WA_LE_IP_GoatV-aGFP_Rep2.tagAlign_density.bw
Input wig:
CEH-34_OP524_WA_LE_Input_Rep0_density.bw
Reads:
The fastq files are the raw data for each replicate.
Rep1 Fastq: 2014-1637_140919_SN484_0310_AC5CHCACXX_1_sequence.txt.gz
Rep2 Fastq: 2014-1638_140919_SN484_0310_AC5CHCACXX_1_sequence.txt.gz
Important files needed to view signal and called peaks in IGB or UCSC Genome Browsers
1) bigBed file to view called peaks in browser (Found in “Optimal IDR thresholded peaks” tab)
spp.optimal.CEH-34_OP524_WA_LE_IP_Rep0.tagAlign_VS_CEH-34_OP524_WA_LE_Input_Rep0.tagAlign.regionPeak.bb
2) wig file (Found in “Control normalized signal” tab) to view signal above input for the pooled replicates
Pooled replicates input subtracted:
CEH-34_OP524_WA_LE_IP_Rep0.tagAlign_VS_CEH-34_OP524_WA_LE_Input_Rep0.tagAlign.bw