Experiment Details Series 20111102_daf-3a_18_L2 Date 2011-11-02 Person pete Treatments none Gene Assayed daf-3 Construct pJIM20::F25E2.5a_1 Imaged Time Points 238 Edited Time Points 135 Edited Cells 359 Comments ubiq EditedBy pete

Strain Details Strain Name RW11524 Date Created 2011-04-06 Source of Genotype stIs11523(daf-3a::H1-Wcherry;unc-119(+));unc-119(tm4063);stIs10024;zuIs178 Reporter Allele stIs11523 Reporter Construct pJIM20::F25E2.5a_1 (Plasmid) Created By DKV/RJT/PW

Construct Details Plasmid Name pJIM20::daf-3a Gene daf-3 Transcript F25E2.5a Promoter Length 5111 Left Primer tggatgactgccacgtaggtaatatttc Right Primer attatgatgatcacccatttatgtgtactctgact Vector pJIM20 Integrated,Expressing Strains 7