Experiment Details
Series
20070806_dpy-7_1
Date
2007-08-06
Person
murray
Treatments
none
Gene Assayed
dpy-7
Construct
pJIM20::F46C8.6.1_1
Imaged Time Points
373
Edited Time Points
190
Edited Cells
180
Comments
arrests after 350 cell stage, doesn"t rotate, expression late (~t325 expression in C hyp, AB hyp?)
EditedBy
JIM
Strain Details
Strain Name
RW10166
Date Created
2007-08-17
Source of Genotype
stIs10157(dpy-7::H1-Wcherry;unc-119(+));unc-119(ed3);stIs10024;zuIs178
Reporter Allele
stIs10157
Reporter Construct
pJIM20::F46C8.6.1_1 (Plasmid)
Created By
EAP/BLM/JIM
Construct Details
Plasmid Name
pJIM20::dpy-7
Gene
dpy-7
Transcript
F46C8.6.1
Promoter Length
2479
Left Primer
ttaatacttgaagatcatgtctacttttccccgattta
Right Primer
ttatctggaacaaaatgtaagaatattcttaaaaattgtttcattcag
Vector
pJIM20
Integrated,Expressing Strains
4