Experiment Details Series 20091216lag-1_L1 Date 2009-12-16 Person zhao Treatments none Gene Assayed lag-1 Construct pJIM20::K08B4.1a_2 Imaged Time Points 274 Edited Time Points 135 Edited Cells 358 Comments n/a EditedBy pete

Strain Details Strain Name RW11138 Date Created 2009-10-30 Source of Genotype stIs10535(lag-1a::H1-Wcherry;unc-119(+));unc-119(ed3);stIs10024;zuIs178 Reporter Allele stIs10535 Reporter Construct pJIM20::K08B4.1a_2 (Plasmid) Created By EAP/DKV/JIM

Construct Details Plasmid Name pJIM20::lag-1a Gene lag-1 Transcript K08B4.1a Promoter Length 2148 Left Primer ttgagtcgtaattgaatttttcaaagcg Right Primer tgagtaggcgagaggcatttcctgaaatttctgaatgt Vector pJIM20 Integrated,Expressing Strains 4