Experiment Details Series 20111220_daf-16f_1_L1 Date 2011-12-20 Person pete Treatments none Gene Assayed daf-16 Construct pJIM20::R13H8.1f_2 Imaged Time Points 255 Edited Time Points 185 Edited Cells 563 Comments n/a EditedBy pete

Strain Details Strain Name RW11615 Date Created 2011-07-06 Source of Genotype stIs11614(daf-16f::H1-Wcherry;unc-119(+));unc-119(tm4063);stIs10024;zuIs178 Reporter Allele stIs11614 Reporter Construct pJIM20::R13H8.1f_2 (Plasmid) Created By DKV/RJT/PW

Construct Details Plasmid Name pJIM20::daf-16f Gene daf-16 Transcript R13H8.1f Promoter Length 3011 Left Primer atatcttggctcttccgtggt Right Primer ggcacttcgagcaatgtaatg Vector pJIM20 Integrated,Expressing Strains 2