Experiment Details Series 20060629_pha4_b2 Date 2006-06-29 Person murray Treatments n/a Gene Assayed pha-4 Construct pJIM20::F38A6.1a_1 Imaged Time Points 370 Edited Time Points 200 Edited Cells 349 Comments 8-40 % red laser D not lineaged EditedBy JIM

Strain Details Strain Name RW10062 Date Created 2006-07-19 Source of Genotype stIs10050(pha-4::H1-Wcherry;unc-119(+));unc-119(ed3);stIs10024;zuIs178 Reporter Allele stIs10050 Reporter Construct pJIM20::F38A6.1a_1 (Plasmid) Created By JIM

Construct Details Plasmid Name pJIM20::pha4_4.1kb Gene pha-4 Transcript F38A6.1a Promoter Length 4100 Left Primer ttagcccggggcccaaattttatgaccaaatga Right Primer tagcctaggtctcaaattagatagtccttcaaaaa Vector pJIM20 Integrated,Expressing Strains 12